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Nucleic Acids Research, 1984, Vol. 12, No. 17 6701-6708
© 1984

ENZYMOLOGY

RsrII-a novel restriction endonuclease with a heptanucleotide recognition site

C.D. O'Connor1,*, E. Metcalf2, C.J. Wrighton1, T.J.R. Harris3 and J.R. Saunders1

1Department of Microbiology, University of Liverpool P.O.Box 147, Liverpool L69 3BX, UK 2P. and S. Biochemicals Ltd. 38 Queensland Street, Liverpool L7 3JG, UK 3Celltech Ltd. 250 Bath Road, Slough SL1 4DY, UK

Received June 28, 1984. Revised August 13, 1984. Accepted August 13, 1984.

A sequence-specific endonuclease present in extracts of Rhodopseudomonas sphaeroides 630 has been purified and characterized. The enzyme, Rsr II, recognises and cleaves the palindromic heptanucleotide sequence:


Formula

By virtue of its unusual specificity, RsrII cuts most DNA molecules very infrequently which should facilitate the physical mapping of large genomes.

*Present address: Department de Biochimie Medicale, Centre Medicale Universitaire, 9 Avenue de Champel, 1211 Geneve 4, Switzerland


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