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Nucleic Acids Research, 1984, Vol. 12, No. 17 6727-6739
© 1984

MOLECULAR BIOLOGY

Analysis of a cloned colicin 1b gene: complete nucleotide sequence and implications for regulation of expression

Jennifer M. Varley and Graham J. Boulnois1

ICI/University Joint Laboratory, University of Leicester Leicester LE1 7RH, UK 1Department of Microbiology, University of Leicester Leicester LE1 7RH, UK

Received June 15, 1984. Revised August 2, 1984. Accepted August 14, 1984.

The complete nucleotide sequence of a 2,971 base pair EcoRI fragment carrying the structural gene for colicin Ib has been determined. The length of the gene is 1,881 nucleotides which is predicted to produce a protein of 626 amino acids and of molecular weight 71,364. The structural gene is flanked by likely promoter and terminator signals and in between the promoter and the ribosome binding site is an inverted repeat sequence which resembles other sequences known to bind the LexA protein. Further analysis of the 5' flanking sequences revealed a second region which may act either as a second LexA binding site and/or in the binding of cyclic AMP receptor protein. Comparison of the predicted amino acid sequence of colicin Ib with that of colicins A and E1 reveals localised homology. The implications of these similarities in the proteins and of regulation of the colicin Ib structural gene are discussed.


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