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Nucleic Acids Research, 1984, Vol. 12, No. 17 6839-6852
© 1984

MOLECULAR BIOLOGY

Genesis of Kirsten murine sarcoma virus: sequence analysis reveals recombination points and potential leukaemogenic determinant on parental leukaemia virus genome

John D. Norton*, Jane Connor+ and Roger J. Avery1

Department of Biological Sciences, University of Warwick Coventry CV4 7AL, UK 1Department of Veterinary Science, Veterinary Research Laboratory, Montana State University Bozeman, MT 59717, USA

Received June 29, 1984. Accepted August 10, 1984.

The genome of Kirsten murine sarcoma virus was formed by recombination between Kirsten murine leukaemia virus seguences, and rat sequences derived from a retrovirus-like ‘30S’ (VL30) genetic element encompassing the Kras oncogene. Using cloned DNAs we have determined the nucleotide sequences of the long terminal repeats and adjacent regions, extending across the points of recombination on the sarcoma and leukaemia virus genomes. Our results suggest that discrete regions of homology and other cryptic sequence features, may have constituted recombinational hot-spots involved in the genesis of the Kirsten murine sarcoma virus genome. We have also compared the sequence of the Kirsten murine leukaemia virus p15 env and adjacent long terminal repeat with the corresponding regions of the AKV and Gross A murine leukaemia virus genomes. This comparison has identified a leukaemogenic determinant in the U3 domain of the long terminal repeat, possibly within a enhancer-like sequence element.

*Present address: Unit of Molecular Genetics, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK

+Present addres: MRC Clinical Research Centre, Watford Road, harrow, Middlesex HA1 3UJ, UK


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