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Nucleic Acids Research, 1984, Vol. 12, No. 17 6853-6859
© 1984

CHEMISTRY

A rapid deprotection procedure for phosphotriester DNA synthesis

T.P. Patel, M.A. Chauncey, T.A. Millican, C.C. Bose and M.A.W. Eaton

Celltech Limited 250 Bath Road, Slough SL1 4DY, UK

Received June 21, 1984. Revised August 13, 1984. Accepted August 13, 1984.

An equimolar solution of aldoxime and tetramethylguanidine at 70°C removes both the base and phosphate protection from oligonucleotides prepared by solid phase phosphate triester technology. The rate of cleavage from the succinyl linkage commonly used for solid phase synthesis is also increased. The method is simpler, faster and nore easily automated than existing methods.


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