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Nucleic Acids Research, 1984, Vol. 12, No. 19 7327-7344
© 1984

MOLECULAR BIOLOGY

The sequence of the gene for cytochrome c oxidase subunit I, a frameshift containing gene for cytochrome c oxidase subunit II and seven unassigned reading frames in Trypanosoma brucei mitochondrial maxi-cirle DNA

Lambert A.M. Hensgens+, Just Brakenhoff, Berend F.De Vries, Paul Sloof, Marijke C. Tromp*, Jacques H. Van Boom* and Rob Benne§

Section for Gene Structure and Expression, Laboratory of Biochemistry, University of Amsterdam P.O. Box 60.000, 1005 GA Amsterdam *Organic Chemistry Laboratory, Gorlaeus Laboratories P.O.Box 9502. 2300 RA Leiden. The Netherlands

§To whom correspondence should be addressed

Received July 30, 1984. Accepted September 13, 1984.

A 9.2 kb segment of the maxi-circle of Trypanosoma brucei mito-chondrial DNA contains the genes for cytochrome c oxidase subunits I and II (coxI and coxII) and seven Unassigned Reading Frames ("URFs").

The genes for coxI and coxII display considerable homology at the aminoacid level (38 and 25%, respectively) to the corresponding genes in fungal and mammalian mtDNA, the only striking point of divergence being an unusually high cysteine content (about 4.5%). The reading frame coding for cytochrome c oxidase subunit II is discontinuous: the C-terminal portion of about 40 amlnoacids, is present in the DNA-sequence in a -1 reading frame with respect to the N-terminal moiety.

URF5, 8 and 10, show a low but distinct homology (about 20%) to mammalian mitochondrial URF-1, 4 and 5, respectively. In URF5, the first AUG is found at codon 145, whereas extensive homology to mammalian URF-1 sequences occurs upstream of this position. The possibility exists that UUG can serve as an initiator codon.

URF7 and URF9 have a highly unusual aminoacid composition and do not possess AUG or UUG initiator codons. These URFs probably do not have a protein-coding function.

The segment does not contain conventional tRNA genes.

+Present address: Istituto di Anatomia Comparata Biologia Generale, Genetica, Via romana 17, Firenze, Italy.


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