Skip Navigation

This Article
Right arrow Print PDF (1022K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (107)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kuwano, R.
Right arrow Articles by Takahashi, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kuwano, R.
Right arrow Articles by Takahashi, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1984, Vol. 12, No. 19 7455-7465
© 1984

MOLECULAR BIOLOGY

Molecular cloning and the complete nucleotide sequence of cDNA to mRNA for S-100 protein of rat brain

Ryozo Kuwano, Hiroshi Usui, Toshinaga Maeda, Toshikazu Fukui*, Nobuko Yamanari*, Eiko Ohtsuka*, Morio Ikehara* and Yasuo Takahashi

Department of Neuropharmacology, Brain Research Institute, Niigata University Asahimachi 1, Niigata 951 *Department of Chemistry, Faculty of Pharmaceutical Sciences, Osaka University 1–6 Yamadaoka, Suita, Osaka 565, Japan

Received August 16, 1984. Accepted September 12, 1984.

The complete nucleotide sequence of mRNA for ß-subunit of rat brain S-100 protein was determined from recombinant cDNA clones. The sequence was composed of 1488 bp which Included the 276 bp of the complete coding region, the 120 bp of the 5'-noncoding region and the 1092 bp of the 3'-noncoding region containing two polyadenylation signals. In addition, the poly(A) tail was also found. The amino add sequence deduced from the nucleotide sequence was homologous to the amino acid sequence of bovine S-100 ß subunit except 4 residues showing species differences. From the viewpoint of evolutionary implications, the homology between the nucleotide sequence of S-100 and those of rat intestinal Ca-b1nding protein (ICaBP) and calmodulin (CaM) was examined. A dot-blot hybridization of poly(A) RNA from the developing rat brainsusing a labeled cDNA showed a rapid increase in S-100 mRNA at 10–20 postnatal days. The presence of S-100 mRNA in C-6 glioma cells 1s also described.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Eur J EndocrinolHome page
W. Allaerts and H. Vankelecom
History and perspectives of pituitary folliculo-stellate cell research
Eur. J. Endocrinol., July 1, 2005; 153(1): 1 - 12.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Nishikawa, I. S. M. Lee, N. Shiraishi, T. Ishikawa, Y. Ohta, and M. Nishikimi
Identification of S100b Protein as Copper-binding Protein and Its Suppression of Copper-induced Cell Damage
J. Biol. Chem., September 12, 1997; 272(37): 23037 - 23041.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.