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Nucleic Acids Research, 1984, Vol. 12, No. 1Part2 569-579
© 1984

MICROCOMPUTERS

Apple II Pascal programs for molecular biologists

Bruno Malthiery1, Bernard Bellon2, Dominique Giorgi3 and Bernard Jacq2,§

1Centre Inter Regional Informatique de Lorraine, Château du Montet Avenue Général Leclerc, 54500 Vandoeuvre Les Nancy 2LGBC-CNRS Luminy case 907, 13288 Marseille Cedex 09 3U 31 INSERM 46 Bd de la Gaye, 13258 Marseille Cedex 09, France

Received August 25, 1983.

A collection of PASCAL programs designed for the Apple II microcomputer is presented. These DNA sequence handling and analysis programs are interactive and may be used even by people with no computer experience.

The package allows the user to enter a sequence from the keyboard, to modify it, to generate the reverse complement, to create new sequences from parts of other ones, to display or print sequences in various formats. Some analysis tasks are also performed : Translation, searches for restriction sites, for homology with subsequences, either perfect or with an adjustable match percentage. In addition, two programs are also included : The first one allows DNA data sequences generated with a BASIC program under the CP/M operating system to be used with these PASCAL programs. The second one is designed for the automatic assembly of DNA fragments sequences, obtained with the GILBERT-MAXAM or M13 techniques, into a complete sequence.

§To whom reprints request should be addressed.


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