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Nucleic Acids Research, 1984, Vol. 12, No. 1Part2 717-729
© 1984

MICROCOMPUTERS

Plasmid mapping computer program

Garry P. Nolan*,*, Claude V. Maina and Aladar A. Szalay+

Boyce Thompson Institute for Plant Research, Cornell University Tower Road, Ithaca, NY 14853, USA

*To whom correspondence concerning details of the program algorithms should be addressed.

Received October 11, 1983.

Three new computer algorithms are described which rapidly order the restriction fragments of a plasmid DNA which has been cleaved with two restriction endonucleases in single and double digestions. Two of the algorithms are contained within a single computer program (called MPCIRC). The Rule-Oriented algorithm, constructs all logical circular map solutions within sixty seconds (14 double-digestion fragments) when used in conjunction with the Permutation method. The program is written in Apple Pascal and runs on an Apple II Plus Microcomputer with 64K of memory. A third algorithm is described which rapidly maps double digests and uses the above two algorithms as adducts. Modifications of the algorithms for linear mapping are also presented.

+To whom reprint requests should be addressed

*Present address: Dept. of Cenetics, Stanford Medical Center, Stanford University, Stanford, CA 94305


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