Skip Navigation

This Article
Right arrow Print PDF (2865K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Adeniyi-Jones, S.
Right arrow Articles by Zasloff, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Adeniyi-Jones, S.
Right arrow Articles by Zasloff, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1984, Vol. 12, No. 2 1101-1115
© 1984

MOLECULAR BIOLOGY

Generation of long read-through transcripts in vivo and in vitro by deletion of 3' termination and processing sequences in the human tRNAimet gene

Samuel Adeniyi-Jones, Paul H. Romeo and Michael Zasloff

Human Genetics Branch, National Institute of Child Health and Human Development, National Institutes of Health Bethesda, MD 20205, USA

Received September 27, 1983. Revised December 1, 1983. Accepted December 1, 1983.

The effects of 3' deletions of the coding and flanking regions of the human Formula gene on its transcription and subsequent processing have been studied both in vitro and in vivo. We demonstrate that in the absence of the oligo T stop signal, polymerase III will read-through efficiently to the next available downstream stop signal. In mutations preserving the 3' terminal sequence of the coding region these read-through transcripts are efficiently processed, irrespective of their length and sequence by an endonucleolytic cleavage to yield both a mature tRNA and an intact trailer RNA. However, deletions involving the terminal regions up to +62 in the coding sequence produce an unprocessed co-transcript of tRNA and downstream sequences. Deletions further within the B promoter box abolish transcription. The use of these mutants as possible "portable" promoters is discussed.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Eukaryot CellHome page
S. Martinez-Calvillo, D. Nguyen, K. Stuart, and P. J. Myler
Transcription Initiation and Termination on Leishmania major Chromosome 3
Eukaryot. Cell, April 1, 2004; 3(2): 506 - 517.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. Chaloin, M. J. Lehmann, G. Sczakiel, and T. Restle
Endogenous expression of a high-affinity pseudoknot RNA aptamer suppresses replication of HIV-1
Nucleic Acids Res., September 15, 2002; 30(18): 4001 - 4008.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. Kuwabara, M. Warashina, S. Koseki, M. Sano, J. Ohkawa, K. Nakayama, and K. Taira
Significantly higher activity of a cytoplasmic hammerhead ribozyme than a corresponding nuclear counterpart: engineered tRNAs with an extended 3' end can be exported efficiently and specifically to the cytoplasm in mammalian cells
Nucleic Acids Res., July 1, 2001; 29(13): 2780 - 2788.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. Koseki, T. Tanabe, K. Tani, S. Asano, T. Shioda, Y. Nagai, T. Shimada, J. Ohkawa, and K. Taira
Factors Governing the Activity In Vivo of Ribozymes Transcribed by RNA Polymerase III
J. Virol., March 1, 1999; 73(3): 1868 - 1877.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
M. Hamada, A. L. Sakulich, S. B. Koduru, and R. J. Maraia
Transcription Termination by RNA Polymerase III in Fission Yeast. A GENETIC AND BIOCHEMICALLY TRACTABLE MODEL SYSTEM
J. Biol. Chem., September 8, 2000; 275(37): 29076 - 29081.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.