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Nucleic Acids Research, 1984, Vol. 12, No. 2 901-914
© 1984

ENZYMOLOGY

DNA binding and antigenic specifications of DNA gyrase

Heinz Lother, Rudi Lurz and Elisha Orr*

Max-Planck-Institut für Molekulare Genetik Ihnestrasse 63–73, D-1000 Berlin 33, FRG Department of Genetics, University of Leicester Leicester LE1 7RH, UK

*To whom correspondence should be addressed

Received November 9, 1983. Accepted November 28, 1983.

Complexes of DNA gyrase and minichromosomal DNA containing the origin of replication of Escherichia coli (oriC) can be formed without metabolic energy and visualised by electron microscopy. The A subunit, part of the A2B2-DNA gyrase complex is the binding protein. Various binding sites are scattered around the minichromosomal DNA including oriC The minimal origin contains the only prominent and reproducible binding site. Binding to this site is suppressed by oxolinic acid and the ATP analogue ß-y-imido ATP. If gyrase isolated from the gram-positive bacterium Bacillus subtilis is used no binding to oriC is seen. This observation is consistent with antigenic differences between the A subunits of the two microorganisms. The binding to oriC might reflect a requirement for DNA gyrase during the initiation of DNA replication.


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