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Nucleic Acids Research, 1984, Vol. 12, No. 2 959-972
© 1984

MOLECULAR BIOLOGY

Nucleotide sequence of the 5'-terminal coding region for pre-rRNA and mature 17S rRNA in Tetrahymena thermophila rDNA

Jan Engberg1, Nanni Din1,*, Hidetoshi Saiga2 and Toru Higashinakagawa3

1Biochem. Inst. B, Univ. Copenhagen 3C Blegdamsvej, DK-2200 Denmark 2Dept. Molecular Biology, Univ. Occupational and Environmental Health Yahata Nishi-Ku, Kitakyusyu 807 3Dept. Biology, Tokyo Metropolitan Univ. Fukazawa Setagaya-Ku, Tokyo 158, Japan

Received October 25, 1983. Accepted December 7, 1983.

The 5'-terminus of 35S pre-rRNA and mature 17S rRNA of Tetrahymena thermophila was mapped on cloned rDNA fragments by S1 nuclease protection experiments. A single site for transcription initiation was observed when pre-rRNA prepared by three different methods was used as RNA probe. These mapping results were unambiguously confirmed by sequencing the 5'-terminus region of in vitro capped 35S pre-rRNA. DNA sequence analysis of about 520 nucleotides upstream of the transcription initiation site revealed several distinct sets of highly conserved repeat sequences. In addition, the 840 nucleotides downstream of the transcription initiation site (+ 1) was determined and shown to include the 5'-terminus of the 17S rRNA coding region at position + 647. A region surrounding the position + 195 contains an inverted repeat sequence which could be the structural basis for the recently described premature transcription termination event in this organism (Kister et al. (1983) Nucl. Acids Res. 11, 3487–3502).

*Present address: Nordisk Gentofte, 1 Niels Steensensvej, DK-2820 Gentofte, Denmark


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