Skip Navigation

This Article
Right arrow Print PDF (2583K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (31)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Sharp, S.
Right arrow Articles by Söll, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sharp, S.
Right arrow Articles by Söll, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1984, Vol. 12, No. 20 7617-7632
© 1984

Articles

Transcriptionally active and inactive gene repeats within the D. meianogaster 5S RNA gene cluster

Stephen Sharp*, Alonzo Garcia, Lynn Cooley+ and Dieter Söll

Institute of Organic Chemistry, Siberian Division of the Academy of Sciences of USSR Lavrent'ev Prospect 9, Novosibirsk 630090, USSR +Institute of Therapy, Siberian Division of the Academy of Medical Sciences of USSR Vladimirovsky spusk 2a, Novosibirsk 630003, USSR

*To whom correspondence should be addressed

Received September 6, 1984. Accepted October 3, 1984.

Transcription of isolated repeat units of D. molanogaster 5S DNA in Drosophila KcO cell extract revealed three types of template activities. 5S1 DNA encodes the known 5S rRNA of D. molanogaster and has a relatively high transcription efficiency. 58II DNA is identical to 5SI DNA except for a two-nucleotide deletion at 5S rRNA positions 28 and 29; the efficiency of transcription is approximately 40% that of 5SI DNA and because of the deletion, the primary transcript is two nucleotides shorter. 5SIII DNA does not support in vitro tranacription (<<2% 5SI DNA), but has the same sequence as 5SI DNA except for a single G to A transition at position 86. This is the first reported point-mutation in a 5S RNA gene reaulting in loss of transcription function. Of approximately 23 5S rRNA gene copies in a cloned 5S DNA sub-cluster (p12D1) 19 appear to be of the transcriptionally inactive 5SIII DNA type.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Genes Dev.Home page
C A O'Brien and S L Wolin
A possible role for the 60-kD Ro autoantigen in a discard pathway for defective 5S rRNA precursors.
Genes & Dev., December 1, 1994; 8(23): 2891 - 2903.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.