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Nucleic Acids Research, 1984, Vol. 12, No. 20 7721-7739
© 1984

Articles

Structural analysis of the two tandemly repeated acid phosphatase genes in yeast

Wajeeh Bajwa*,+, Bernd Meyhack*, Hans Rudolph*, Anne-Marie Schweingruber1 and Albert Hinnen*

Friedrich Miescher Institut, Postfach 2543, CH-4002 Basel, Switzerland 1Institute for General Microbiology Baltzer-Strasse 4, CH-3012 Bern, Switzerland

+To whom correspondence and reprint requests should be addressed

Received July 13, 1984. Revised September 5, 1984. Accepted September 24, 1984.

We have sequenced the genetically linked genes for repressible (PH05) and and constitutive (PH03) acid phosphatase from S. cerevisiae Both genes are located on a 3.91 Kb BamHI and HpaI fragment, in the order (5') PH05. PH03 (3'). The mRNA transcripts have been analysed by S1-nuclease mapping. They show heterogenous initiation sites. Each of the PH05 and PH03 genes codes for 467 amino acids as deduced from the DNA sequence. The coding regions of the two genes show homology both at the nucleotide (82%) and the amino acid (87%) level. In the coding sequences, long stretches of homologous regions are flanked by small non-homologous regions. The nucleotide homology (65%) extends to some length into the 5' and 3' non-coding flanking sequences. Further upstream sequences are unrelated.

The comparison of the NH2-terminal amino acid sequence deduced from the nucleotide sequence, with that of purified repressible acid phosphatase revealed the presence of a putative signal peptide.

*Present address: Biotechnology Department, CIBA-GEIGY AG, CH-4002 Basel, Switzerland


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