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Nucleic Acids Research, 1984, Vol. 12, No. 20 7811-7830
© 1984

Articles

Tandem promoters determine regulation of the Klebsiella pneumoniae glutamine synthetase (glnA) gene

Ray Dixon

AFRC Unit of Nitrogen Fixation, University of Sussex, Brighton BN1 9RQ, UK

Received August 14, 1984. Revised September 27, 1984. Accepted September 27, 1984.

Transcription of the structural gene for glutamine synthetase(glnA) in Klebsiella pneuxnoniae is controlled by the nitrogen regulatory genes ntrA, ntrB and ntrC The nucleotide sequence of the regulatory region upstream of the ginA gene is reported here. High resolution S1 mapping of in vivo transcripts indicates that the regulatory region contains tandem promoters separated by 100 nucleotides. Measurements ofß-galactosidase activities determined in vivo from glnA-lac fusions suggest that the upstream promoter (for RNA2) is negatively regulated by the ntrBC gene products whereas transcription from the downstream promoter (for RNA1) is positively activated by the ntrA gene product in the presence of either the ntrBC or the nifA genes. The nucleotide sequence of the up stream promoter resembles the consensus sequence for E. coli promoters, whereas the downstream promoter shows homology with the nitrogen fixation (nif) promoters of K. pneumoniae.


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