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Nucleic Acids Research, 1984, Vol. 12, No. 20 7831-7846
© 1984

Articles

A functional map of the nopaline synthase promoter

C.H. Shaw, G.H. Carter, M.D. Watson and C.H. Shaw*

Department of Botany, University of Durham, Science Laboratories South Road, Durham DH1 3LE, UK

*To whom all correspondence should be addressed

Received August 13, 1984. Revised September 25, 1984. Accepted September 25, 1984.

This paper describes the first functional map of a promoter expressed from the plant chromosome. We have constructed a series of overlapping deletion mutants within the region upstream of the Ti-plasmid encoded nopaline synthase (nos) gene. By monitoring nos expression in tumour tissue we have inferred a functional map of the nos promoter. The maximum length of sequence upstream of the transcription initiation point required to express wild type levels of nopaline synthase is 88 bp. Within this region, the "CAAT" box is essential for maximal activity; deletion of this sequence reduced apparent nos expression by over 80%. Presence of an intact or partial "TATA" box in the absence of the "CAAT" box supports a barely detectable level of nopaline synthase. Removal of all sequences upstream of the nos coding sequence results in no detectable activity.


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