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Nucleic Acids Research, 1984, Vol. 12, No. 20 7915-7927
© 1984

Articles

Conformations of poly(dG-dC).poly(dG-dC) modified by the O-acetyl derivative of the carcinogen 4-hydroxyaminoquinoliue 1-oxide

B. Bailleul, S. Galiègue-Zouitina and M.H. Loucheux-Lefebvre

Unité INSERM no 124, Institut de Recherches sur le Cancer de Lille, Place de Verdun, 59045 Lille Cédex, France

+To whom reprint requests should be addressed

Received July 25, 1984. Revised September 28, 1984. Accepted September 28, 1984.

Poly(dG-dC)·poly(dG-dC) has been modified by reaction with 4-acetoxyaminoquinoline 1-oxide (Ac-4 HAQO), the ultimate carcinogen of 4-nitroquinoline 1-oxide. The circular dichroism (CD) spectra of the modified and unmodified polymers have been compared under various experimental conditions. The CD spectra were recorded in 1 mM phosphate, 50% (v/v) ethanol, 3.8 M LiCl and 95% (v/v) ethanol, conditions in which poly(dG-dC).poly(dG-dC) adopts the B-, Z-, C- and A-form respectively. In 1 mM phosphate buffer, poly(dG-dC).poly(dG- dC) modified by Ac-4 HAQO seems not to contain regions in the Z-form. Z-form induction could be progressively obtained by the addition of ethanol as follows : in the buffer with about 30% ethanol the modified polymer started to adopt the Z structure, while 40% of ethanol in the buffer was necessary for the unmodified polymer. In the 50% ethanol-1 mM phosphate buffer mixture (v/v), poly(dG was entirely in the Z-forin while poly(dG-dC). poly(dC-dC) modified by Ac-4 HAQO remained partially in the B-form.

Eazymatic digestions with the nuclease S1 which is specific of the single-stranded DNA were carried out in order to support the modified poly(dG-dC). poly(dG-dC) CD study conclusions. The role played by the two major adducts on the conformational characteristics of modified polymer is discussed.


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