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Nucleic Acids Research, 1984, Vol. 12, No. 21 7975-7985
© 1984

MOLECULAR BIOLOGY

Yeast may not contain histone H1: the only known ‘histone H1-like’ protein in Saccharomyces cerevisiae is a mitochondrial protein

Ulrich Certa, Mary Colavito-Shepanski and Michael Grunstein

Molecular Biology Institute and Department of Biology, University of California Los Angeles, CA 90024, USA

Received August 27, 1984. Revised September 28, 1984. Accepted October 10, 1984.

It is likely that histone H1 is involved in the condensation of chromatin in eukaryotes. However, both the presence of histone H1 in yeast and the extent of yeast chromatin condensation are controversial. A 20 kD protein copurifies with yeast chromatin and was shown by other investigators to have characteristics of histone H1 protein. In an attempt to obtain a positive Identification of the 20 kD protein, we purified the protein to homogeneity and raised antibodies against it. We show here by immunofluorescence that the 20 kD protein does not localize to the nucleus but to cytoplasmic particles resembling mitochondria. Furthermore, we show by Western-blot analysis that anti-20 kD protein antibodies react to protein isolated from purified mitochondria. Finally, we present evidence based on size, charge, amino acid composition and immunological cross reactivity to suggest that the yeast 20 kD protein is likely to be the mitochondrial DNA-binding HM protein. This leaves no candidate for histone H1 1n yeast.


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