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Nucleic Acids Research, 1984, Vol. 12, No. 21 8097-8114
© 1984

MOLECULAR BIOLOGY

Increased abundance of a normal cell mRNA sequence accompanies the conversion of rat mammary cuboidal epithelial cells to elongated myoepithellal-llke cells in culture

R. Barraclough*, R. Kimbell and P.S. Rudland

Ludwig Institute for Cancer Research (London Branch), Royal Marsden Hospital Sutton, Surrey, UK

*Address for correspondence: Dr.R.Barradough, Ludwig Institute for Cancer Research, Haddow Laboratories, Clifton Avenue, Sutton, Surrey SM2 5PX, UK

Received July 12, 1984. Revised September 5, 1984. Accepted October 8, 1984.

Rat mammary cuboidal epithelial cell lines in culture convert to elongated myoepithelial-like cells. This conversion is accompanied by the appearance of a 9,000 molecular weight acidic polypeptide (p9ka), abundant in the elongated convertants, but which is hardly detectable in the cuboidal epithelial cells. A cDNA library corresponding to a low-molecular-weight fraction of poly(A)- containing RNA from a myoepithelial -like cell line, has been constructed. Recombinant plasmids containing cDNA complementary to p9ka mRNA have been identified by hybrid-selected translation. The mRNA for p9ka has been identified by Northern blotting and is found to be at least five-times more abundant in cultured myoepithelial -like rat mammary cells when compared to the cuboidal epithelial cells. This cytoplasmic mRNA sequence, which 1s present in increased abundance in cultured mammary myoepithelial -like cells, is also present, at lower levels, in normal rat tissues, including the mammary glands.


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