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Nucleic Acids Research, 1984, Vol. 12, No. 21 8129-8144
© 1984

MOLECULAR BIOLOGY

Nucleic acid sequence and chromosome assignment of a wheat storage protein gene

Olin D. Anderson, James C. Litts, Marie-Francoise Gautier1 and Frank C. Greene

Food Proteins Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Western Regional Research Center Albany, CA 94710, USA

Received May 30, 1984. Revised September 28, 1984. Accepted September 28, 1984.

A cloned gliadin gene was isolated from a wheat genomic library, and 2.4 kb of its primary sequence determined. The gene, {alpha}-1Y, was found by Southern analysis to be located on chromosome 6A, and its derived amino acid sequence identifies it as a member of the A-gliadin subgroup of {alpha}-gliadins located on the short arm of that chromosome. {alpha}–1Y is apparently functional, and contains consensus TATA and CAAT boxes, and poly-adenylatlon signals. This gliadin gene has no introns, and its noncoding flanking regions contain several short repeats and inverted sequences. The gene is contained in a 6.2 kb EcoRI genomic fragment whose apparent copy number varies in different wheat cultivars.

1Permanent address: Laboratoire de Technologic des Cereales, Institut National de la Recherche Agronomique, 9 Place Viala, 34060 Montpellier, France


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