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Nucleic Acids Research, 1984, Vol. 12, No. 22 8407-8414
© 1984

MOLECULAR BIOLOGY

Molecular cloning of a human immunoglobulin {lambda} chain variable sequence

Y. Tsujimoto and C.M. Croce

Wistar Institute of Anatomy and Biology 36th Street at Spruce, Philadelphia, PA 19104, USA

Received July 30, 1984. Revised October 17, 1984. Accepted October 17, 1984.

We have cloned a human V{lambda} cDNA sequence from an Ig{lambda}-producing human Burkitt lymphoma cell line (BL2) by taking advantage of a cloned constant region gene as a primer for cDNA synthesis instead of an oligo(dT) primer. The amino acid sequence deduced from the nucleotide sequence of V{lambda} clones is highly related to that of the NEW V{lambda} protein of subgroup I. Southern blot hybridization of human DMAs with the V{lambda}I probe showed at least 12 hybridizing V{lambda} fragments. These fragments are amplified in K562 cells which derive from a case of chronic myelogenous leukemia and contain an amplified c-abl oncogene and amplified C{lambda} sequences.


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K Kawasaki, S Minoshima, K Schooler, J Kudoh, S Asakawa, P J de Jong, and N Shimizu
The organization of the human immunoglobulin lambda gene locus.
Genome Res., September 1, 1995; 5(2): 125 - 135.
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