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Nucleic Acids Research, 1984, Vol. 12, No. 22 8415-8436
© 1984

MOLECULAR BIOLOGY

Only three of the seven human chorionic gonadotropin beta subunit genes can be expressed in the placenta

Karen Talmadge1,*,+, William R. Boorstein2,*, Nikos C. Vamvakopoulos3,*, Mary-Jane Gething* and John C. Fiddes1,*,+

*Cold Spring Harbor Laboratory P.O. Box 100, Cold Spring Harbor, NY 11724 +California Biotechnology, Inc. 2450 Bayshore Frontage Road, Mountain View, CA 94043, USA

Received July 25, 1984. Revised October 16, 1984. Accepted October 16, 1984.

Human chorionic gonadotropin (hCG) is a placental hormone essential for the maintenance of pregnancy. While the alpha subunit of this hormone is encoded by a single gene, the beta subunit is encoded by a complex family of seven very similar genes or pseudogenes. Two approaches have been taken to establish which of these genes are functional. First, we have used two restriction enzyme site polymorphisms to correlate 15 independently isolated beta hCG cDNA clones with their corresponding genes. Second, we have used transient expression in COS cells to assay for correctly-initiated transcription from six of the seven beta hCG gene promoters. From these data, we conclude that, at most, only three of the seven beta hCG genes are expressed in the placenta. Comparison of the sequences of a functional and a non-functional beta hCG gene reveals no obvious differences, such as promoter changes, that could account for this differential expression.

1Present address: California Biotechnology, Inc., 2450 Bayshore Frontage Rd., Mountain View, CA 94043 USA

2Present address: Department of Molecular Biology, Madison, WI353706 USA

3Present address: 28 Kononos, Pagrati, Athens, Greece


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