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Nucleic Acids Research, 1984, Vol. 12, No. 22 8553-8566
© 1984

CHEMISTRY

Conformational changes induced in DNA by the in vitro reaction with the mutagenic amine: 3-N,N-acetoxyacetylainnio-4,6-dimethryldipyrido (l,2-a:3',2'-d) imidazole

Eric Hébert, Bernard Loukakou, Germain Saint-Ruf and Marc Leng

Centre de Biophysique Moléculaire CNRS, 1A, avenue de la Recherche Scientifique, 45045 Orléans Cédex, France

Received September 13, 1984. Revised October 25, 1984. Accepted October 25, 1984.

The conformation of synthetic or natural DNAs modified in vitro by covalent binding of N-AcO-A-Glu-P-3 was investigated by fluorescence and circular dichroism. In all cases, substitution occurs mainly on the C8 of guanine residues. In modified poly(dG-dC).poly(dG-dC) or poly(dA-dC).poly(dG-dT) in B conformation, A-Glu-P-3 residues interact strongly with the bases whereas in Z conformation these residues are largely exposed to the solvent and interact weakly with the bases. A-Glu-P-3 and N-acetyl-2-aminofluorene (AAF) residues are equally efficient to induce the B-Z transition of poly(dG-dC).poly(dG-dC) and of poly(dA-dC).poly(dG-dT). Modifications of poly(dG).poly(dC) and calf thymus DNA indicate strong interactions between A-Glu-P-3 and the bases.


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