Nucleic Acids Research, 1984, Vol. 12, No. 23 8779-8799
© 1984
MOLECULAR BIOLOGY |
Stimulation of in vitro transcription by the upstream element of the adenovirus-2 major late promoter involves a specific factor
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologic Moléculaire et de Génie Geénétique de I'INSERM, Institut de Chimie Biologique Faculté de Medecine, 11, rue Humann, 67085 Strasbourg Ceédex, France
*To whom reprint requests should be sent
Received October 24, 1984. Accepted November 8, 1984.
We have previously reported that sequences located upstream from the TATA box of the Adenov1rus-2 major late promoter (Ad2MLP), between –34 and –97, are necessary for efficient transcription in vivo and in vitro (1). We have utilized an in vitro competition assay to demonstrate that the upstream element requirement involves the binding of a specific factor(s), which results in stimulation of in vitro transcription from the Ad2MLP. DNA fragments prepared from Ad2MLP upstream sequence mutants which are transcribed much less efficiently than the wild-type promoter both in vivo and in vitro were shown to be unable to bind this factor. We have also constructed chimeric promoter recombinants containing the 21bp repeat upstream element of the SV40 early promoter inserted upstream from the Ad2MLP TATA box. The SV40 upstream element stimulates in vitro transcription from the heterologous Ad2MLP TATA box element; competition experiments show that the Ad2MLP upstream element-specific factor is different from the SV40-specific factor Sp1.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. E. Anthony, H. A. Mason, T. Gridley, G. Fishell, and N. Heintz Brain lipid-binding protein is a direct target of Notch signaling in radial glial cells Genes & Dev., May 1, 2005; 19(9): 1028 - 1033. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Okada, E. Matsuura, Z. Tozuka, R. Nagai, A. Watanabe, K. Matsumoto, K. Yasui, R. W. Jackman, T. Nakano, and T. Doi Upstream stimulatory factors stimulate transcription through E-box motifs in the PF4 gene in megakaryocytes Blood, October 1, 2004; 104(7): 2027 - 2034. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Bradsher, F. Coin, and J.-M. Egly Distinct Roles for the Helicases of TFIIH in Transcript Initiation and Promoter Escape J. Biol. Chem., January 28, 2000; 275(4): 2532 - 2538. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. B. Arnold and N. Heintz A calcium responsive element that regulates expression of two calcium binding proteins in Purkinje cells PNAS, August 5, 1997; 94(16): 8842 - 8847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P D Gregor, M Sawadogo, and R G Roeder The adenovirus major late transcription factor USF is a member of the helix-loop-helix group of regulatory proteins and binds to DNA as a dimer. Genes & Dev., October 1, 1990; 4(10): 1730 - 1740. [Abstract] [PDF]
|
|
|
|
|
|
J Carcamo, E Maldonado, P Cortes, M H Ahn, I Ha, Y Kasai, J Flint, and D Reinberg A TATA-like sequence located downstream of the transcription initiation site is required for expression of an RNA polymerase II transcribed gene. Genes & Dev., September 1, 1990; 4(9): 1611 - 1622. [Abstract] [PDF]
|
|
|
|
|
|
F Watt and P L Molloy Cytosine methylation prevents binding to DNA of a HeLa cell transcription factor required for optimal expression of the adenovirus major late promoter. Genes & Dev., September 1, 1988; 2(9): 1136 - 1143. [Abstract] [PDF]
|
|