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Nucleic Acids Research, 1984, Vol. 12, No. 23 8801-8818
© 1984

MOLECULAR BIOLOGY

The MLV and SV40 enhancers have a similar pattern of transcriptional activation

P. Augereau* and B. Wasylyk

Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de I'INSERM, Institut de Chimie Biologique Faculté de Médecine, 11, rue Humann, 67085 Strasbourg Cédex, France

*To whom reprint requests should be sent

Received November 10, 1984. Accepted November 13, 1984.

Activation of transcription by the Moloney Murine Leukemia Virus (MLV) and Simian Virus (SV40) enhancers was compared by transfecting recombinants containing these enhancers in either mouse or human cell-lines, and analysing RNA 48 h later by quantitative S1 nuclease mapping. The enhancers share the following properties. They stimulate transcription 1n an orientation-independent manner from the same startsites on the natural heterologous conalbumin (+62 to –102) or SV40 early promoter elements as well as on substitute promoter elements. The enhancers are most efficient when they are located directly upstream from the conalbumin (+62 to –102) promoter element, but they still stimulate transcription when they are either immediately downstream from the promoter element, or further upstream. Increasing the distance by interposing DNA sequences between the enhancers and the conalbumin promoter fragment results in decreased activation. Both enhancers show some cell-line specificity for activation of transcription. However, in all cell-lines and constructions tested the MLV enhancer was always less efficient than the SV40 enhancer. These results suggest that the MLV and SV40 enhancers stimulate transcription by similar mechanisms.


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