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Nucleic Acids Research, 1984, Vol. 12, No. 23 8835-8846
© 1984

MOLECULAR BIOLOGY

A complete and a truncated U1 snRNA gene of Drosophila melanogaster are found as inverted repeats at region 82E of the polytene chromosomes

J. Kejzlarová-Lepesant1, Hugh W. Brock2, Jacques Moreau1, Marie-Liesse Dubertret1, Alain Billault1 and J-A. Lepesant1,*

1Institut J. Monod, Tour 43, CNRS et Université Paris VII, 2 Place Jussieu 75251 Paris Cedex 5, France 2Department of Zoology, University of British Columbia 6270 University Blvd., Vancouver V6T 2A9, Canada

*To whom reprint requests should be sent.

Received September 27, 1984. Revised November 7, 1984. Accepted November 7, 1984.

A phage containing two sequences homologous to U1 snRNA was isolated from a Drosophila melanogaater genomic library, and identified with a previously cloned D. melanoqaster Ul anRNA gene. DNA sequence analysis showed that complete and truncated Ul snRNA genes are present, both of which have base substitutions relative to Ul snRNA. These genes show conservation of 5' and 3' flanking regions relative to other Ul and U2 snRNA genes of Drosophila. Intramolecular renaturation experiments and electron microscope mapping demonstrates that the two Ul snRNA sequences are present as inverted repeats about 2.7kb apart, separated by a smaller pair of inverted repeats of an unrelated sequence. These Ul snRNA sequences were located by in situ hybridization at 82E, and related sequences were found at 21D and 95C on the polytene chromosome map.The results are discussed with reference to the origin and function of snRNAa.


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