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Nucleic Acids Research, 1984, Vol. 12, No. 23 8917-8925
© 1984

MOLECULAR BIOLOGY

Chloroplast promoter driven expression of the chloramphenicol acetyl transferase gene in a cyanobacterium

Valdis A. Dzelzkalns, Geoffrey C. Owens and Lawrence Bogorad

Biological Laboratories, Harvard University 16 Divinity Avenue, Cambridge, MA 02138, USA

Received September 4, 1984. Revised November 5, 1984. Accepted November 5, 1984.

The putative promoter region of the chloroplast encoded ps2B gene (the gene encoding the 32kD herbicide binding B protein of photosystem II (1–4)) has been fused to a chloramphenicol acetyl transferase (CAT) gene that lacks its bacterial promoter and found to accurately initiate transcription from this promoter when introduced into the cyanobacterium, Anacystis nidulans R2 (or into E. coli). The chloroplast promoter-CAT fusion was introduced into the cells on a plasmid that contains plasmid replication origins for E. coli and Anacystis as well as a second antibiotic resistance marker. Cells transformed with corresponding vectors lacking the promoter region do not express CAT.


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