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Nucleic Acids Research, 1984, Vol. 12, No. 23 8951-8970
© 1984

MOLECULAR BIOLOGY

Homologous versos heterologous gene expression in the yeast, Saccharomyces cerevisiae

Christina Y. Chen, Hermann Oppermann and Ronald A. Hitzeman

Department of Molecular Biology, Genentech, Inc. 460 Point San Bruno Boulevard, South San Francisco, CA 94080, USA

Received August 24, 1984. Revised November 9, 1984. Accepted November 9, 1984.

DNA sequences normally flanking the highly expressed yeast 3-phospho-glycerate kinase (PGK) gene have been placed adjacent to heterologous mammalian genes on high copy number plasmid vectors and used for expression experiments in yeast. For many genes thus far expressed with this system, expression has been 15–50 times lower than the expression of the natural homologous PGK gene on the same plasmid. We have extensively investigated this dramatic (Difference ana have found that in most cases it is directly proportional to the steaay-state levels of mRNAs. We demonstrate this phenomenon and suggest possible causes for this effect on mRNA levels.


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