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Nucleic Acids Research, 1984, Vol. 12, No. 23 9025-9038
© 1984

MOLECULAR BIOLOGY

Chromatin structure at the 44D larval cuticle gene locus in Drosophila: the effect of a transposable element insertion

Joel C. Eissenberg, Deborah A. Kimbrell1, James W. Fristrom1 and Sarah C.R. Elgin

Department of Biology, Washington University St. Louis, MO 63130 1Department of Genetics, University of California Berkeley, CA 94720, USA

Received July 16, 1984. Revised November 6, 1984. Accepted November 6, 1984.

The chromatin structure of the larval cuticle gene cluster at 44D was characterized in embryos from wild-type (Oregon R) and a variant line (27sol;3) of Drosophila melanogaster. A major DNase I hypersensitive (DH) site was found between genes II and III in the chromatin, in a position 5' to the transcriptional start of the genes in the cluster. The introduction of a 7.3 kilobase transposable element into the cluster in the 2/3 variant enhanced the sensitivity of the major site in 2/3 chromatin but had no other effect upon the pattern of DH sites associated with the wild-type sequences. The wild-type sequences were packaged into an ordered nucleosome-like array in embryos, as revealed by digestion with the chemical cleavage reagent (methidiumpropyl-EDTA) iron (II) [MPE.Fe(II)]. Nucleolytic cleavage within the transposable element chromatin shows it to be organized in an ordered array punctuated by several DH sites. While the patterns of DNase I hyper-sensitivity are similar in the vicinity of the direct terminal repeats, the patterns revealed by micrococcal nuclease and MPE.Fe(II) are not, Indicating a different chromatin organization of these two identical sequences.


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