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Nucleic Acids Research, 1984, Vol. 12, No. 23 9191-9204
© 1984

MOLECULAR BIOLOGY

The chromatin structure of the human {varepsilon} globin gene: nuclease hypersensitive sites correlate with multiple Initiation sites of transcription

Jing-de Zhu*, Maggi Allan+ and John Paul+

Beatson Institute for Cancer Research Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK

+To whom correspondence should be addressed

Received July 24, 1984. Revised October 30, 1984. Accepted October 30, 1984.

We have mapped sites in chromatin flanking the {varepsilon}-globin gene in the K562 cell which are hypersensitive to digestion with DNAseI, micrococcal nuclease and S1 nuclease. Many of those in the 5' flanking region correspond to minor upstream transcriptional starts. However, one prominent site occurs upstream of the boundary of transcription; it maps to a region with an unusual DNA sequence. In baby hamster kidney cells stably transformed with recombinant DNA containing the human {varepsilon}-globin gene and in Cos 7 cells transiently transfected with DNA containing the {varepsilon}-globin gene, hypersensitive sites can be demonstrated.

*Permanent Address: Shanghai Institute for Cell Biology, 320 Yo.-Yang Road, Shanghai, China


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