Skip Navigation

This Article
Right arrow Print PDF (1227K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (234)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Fox, K. R.
Right arrow Articles by Waring, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fox, K. R.
Right arrow Articles by Waring, M. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1984, Vol. 12, No. 24 9271-9285
© 1984

MOLECULAR BIOLOGY

DNA structural variations produced by actinomycin and distamycin as revealed by DNAase I footprinting

Keith R. Fox and Michael J. Waring

Department of Pharmacology, University of Cambridge, Medical School Hills Road, Cambridge CB2 2QD, UK

Received October 26, 1984. Accepted November 23, 1984.

The technique of DNAase I footprinting has been used to investigate preferred binding sites for actinomycin D and distamycin on a 160-base-pair DNA fragment from E.coli containing the tyr T promoter sequence. Only sites containing the dinucleotide step GpC are protected by binding of actinomycin, and all such sites are protected. Distamycin recognizes four major regions rich in A+T residues. Both antibiotics induce enhanced rates of cleavage at certain regions flanking their binding sites. These effects are not restricted to any particular base sequence since they are produced in runs of A and T by actinomycin and in GC-rich sequences by distamycin. The observed increases in susceptibility to nuclease attack are attributed to DNA structural variations induced in the vicinity of the ligand binding site, most probably involving changes in the width of the helical minor groove.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
X. Shi and J. B. Chaires
Sequence- and structural-selective nucleic acid binding revealed by the melting of mixtures
Nucleic Acids Res., January 23, 2006; 34(2): e14 - e14.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Roque, M. Orrego, I. Ponte, and P. Suau
The preferential binding of histone H1 to DNA scaffold-associated regions is determined by its C-terminal domain
Nucleic Acids Res., November 23, 2004; 32(20): 6111 - 6119.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Widlak, O. Palyvoda, S. Kumala, and W. T. Garrard
Modeling Apoptotic Chromatin Condensation in Normal Cell Nuclei. REQUIREMENT FOR INTRANUCLEAR MOBILITY AND ACTIN INVOLVEMENT
J. Biol. Chem., June 7, 2002; 277(24): 21683 - 21690.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Y. Hsu and Y.-H. Wang
Human Fragile Site FRA16B DNA Excludes Nucleosomes in the Presence of Distamycin
J. Biol. Chem., May 3, 2002; 277(19): 17315 - 17319.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. Sera and A. P. Wolffe
Role of Histone H1 as an Architectural Determinant of Chromatin Structure and as a Specific Repressor of Transcription on Xenopus Oocyte 5S rRNA Genes
Mol. Cell. Biol., July 1, 1998; 18(7): 3668 - 3680.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
G. K. Geiss, C. A. Radebaugh, and M. R. Paule
The Fundamental Ribosomal RNA Transcription Initiation Factor-IB (TIF-IB, SL1, Factor D) Binds to the rRNA Core Promoter Primarily by Minor Groove Contacts
J. Biol. Chem., November 14, 1997; 272(46): 29243 - 29254.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
P. Dervan
Design of sequence-specific DNA-binding molecules
Science, April 25, 1986; 232(4749): 464 - 471.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.