Skip Navigation

This Article
Right arrow Print PDF (4091K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (28)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Back, E.
Right arrow Articles by Tobler, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Back, E.
Right arrow Articles by Tobler, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1984, Vol. 12, No. 3 1313-1332
© 1984

MOLECULAR BIOLOGY

Structural organization of the two main rDNA size classes of Ascaris lumbricoides

Eduard Back, Müller Fritz* and Heinz Tobler+

Zoologisches Institut der Universität Freiburg Pérolles, CH-1700 Freiburg, Switzerland

+To whom correspondence and reprint requests should be addressed

Received December 6, 1983. Accepted January 6, 1984.

The two main rDNA size classes in the genome of Ascaris lumbricoides consist of 8.8 kb and 8.4 kb long repeating units present in a quantitative ratio of roughly 10:1. They both contain the genes coding for l8S, 5.8S and 26S ribosomal RNAs. The length heterogeneity is due to a 450 bp long spacer region localized in the longer repeating unit which begins 870 bp upstream of the 5'-end 18S gene. A few additional microheterogeneities in base sequence occur at the 5'-end of the 26S gene. The 18S, 5.8S and 26S coding regions have been mapped on both the 8.8 kb and 8.4 kb repeating units and the localization of the 5'- and 3'-ends of the 18S and 26S genes has been performed by S1 protection. No intervening sequences are present in either coding region of the two main rDNA size classes.

*Present address: Department of Embryology, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, Maryland 21210.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
R. E. Davis, A. Parra, P. T. LoVerde, E. Ribeiro, G. Glorioso, and S. Hodgson
Transient expression of DNA and RNA in parasitic helminths by using particle bombardment
PNAS, July 20, 1999; 96(15): 8687 - 8692.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.