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Nucleic Acids Research, 1984, Vol. 12, No. 3 1517-1528
© 1984

MOLECULAR BIOLOGY

Intracellular forms of viral DNA consistent with a model of reverse transcriptional replication of the cauliflower mosaic virus genome

Yves Marco and Stephen H. Howell*

Biology Department CO 16, University of California San Diego, La Jolla, CA 92093, USA

*Address all correspondence to: Dr. Stephen H.Howell, c/o Laboratory of Dr. W.J.Peacock, CSIRO, Division of Plant Industry, P Box 1600, Canberra City, ACT 2601, Australia

Received July 27, 1983. Revised December 15, 1983. Accepted December 15, 1983.

Five unencapsidated, intracellular forms of cauliflower mosaic virus DNA which accinulate in infected turnip leaves are described. The forms are double stranded (or partially double stranded), and one (form iv) is a covalently-closed circular form of the full-length genane. Form i is an open form composed of full-length genome strands (7.6 kilobases) similar to encap- sidated viral DNA. Form iii appears as a diffuse band on gels and is composed of two approximately half length strands (~3.8 kb) spanning the region of the genome between the twe site-specific DNA breaks ({Delta}1 and {Delta}2)Form ii is composed of a full length strand and an approximately half-length strand as described for form iii DNA. Form v is a small form (~0.7 kilobases in undenatured form) that maps adjacent to the {alpha}-strand break ({Delta}1 and may be a fold- back form. These forms appear to be intermediates in cauliflower mosaic virus DNA replication and the properties of these forms are consistent with possible intermediates in a model of reverse transcriptional replication of the viral genome.


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