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Nucleic Acids Research, 1984, Vol. 12, No. 3 1563-1572
© 1984


MOLECULAR BIOLOGY

Lysogenization of ultraviolet-irradiated Escherichia coli with {lambda}: dependence on the recA and recB gene products

Erika Salaj-Smic, Drasko Serman*, maja Vlahovic*, Mirjana Petranovic, Drago Petranovic and Zeljko Trgovcevic

Department of Experimental Biology and Medicine, Institute ‘Rudjer Boskovic 41001 Zagreb, Croatia, Yugoslavia *Department of Biology, Medical Faculity, University of Zagreb 41001 Zagreb, Croatia, Yugoslavia

Received December 6, 1983. Accepted January 6, 1984.

The lysogenization of ultraviolet-irradiated Escherichia coli cells by the {lambda}. phage was studied. Genetic analysis indicates that changes in the number of the lysogenized cell during post-UV growth is primarily due to the change in the proteolytic activity of RecA protein. Full proteolytic activity is achieved only in the presence of the functional recB gene product.


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