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Nucleic Acids Research, 1984, Vol. 12, No. 3 1671-1686
© 1984

ENZYMOLOGY

2', 3'-Dideoxy-3' amlnonudeo 5' .triphosphates are the terminators of DNA synthesis catalyzed by DNA polymerases

Z.G. Chidgeavadze, R.Sh. Beabealashvilli, A.M. Atrazhev, M.K. Kukhanova, A.V. Azhayev and A.A. Krayevsky

National Cardiology Research Center of the USSR Academy of Medical Sciences 3-rd Cherepkovskaya str., 15A Moscow 121522, USSR Institute of Molecular Biology of the USSR Academy of Sciences Vavilov str., 32, Moscow 117984, USSR

Received October 20, 1983. Accepted December 14, 1983.

It is shown that 2',3'-dideoxy-3'-aminonucleoside 5'-tri-phosphates with adenine, guanine, cytosine and thymine bases are effective inhibitors of DNA polymerase I, calf thymus DNA polymerase {alpha} and rat liver DNA polymerase ß. The effect of the above-mentioned compounds is markedly higher than corresponding action of the well-known DNA synthesis inhibitors arabinonucleoside 5'-triphosphates and 2',3'-dideoxynucleoside 5'-triphosphates. 2', 3' -dideoxy-3' -aminonucleoside 5'-monophosphate residues incorporate into the 3'-terminus of the primer and terminate the DNA chain elongation. The possibility of using 2',3'-dideoxy-3'- aminonucleoside 5'-triphosphates as terminators for DNA sequencing by the polymerization method is demonstrated.


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