Skip Navigation

This Article
Right arrow Print PDF (3087K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Ramakrishna, N.
Right arrow Articles by Smith, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ramakrishna, N.
Right arrow Articles by Smith, I.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1984, Vol. 12, No. 4 1779-1790
© 1984

MOLECULAR BIOLOGY

The complele DNA sequence and regulatory regions of the Bacillus licheniformis spoOH gene

N. Ramakrishna, E. Dubnau and I. Smith

Department of Microbiology, Public Health Research Institute of the City of New York, Inc. 455, First Avenue, New York, NY 10016, USA

Received January 9, 1983. Accepted January 20, 1984.

We have determined the sequence of a 1228 base-pair cloned DNA fragment from Bacillus licheniformis capable of specifically complementing mutations in the spoOH gene, which is required for the early stage of sporulation in B. subtilis The sequence has only one long open reading frame consisting of l68 codons. In vivo and in vitro transcription mapping studies indicate the size of complemantory RNA to be around 1 kb with the 5' initiation site at base 79 and the 3' termination site in the area of base 1138. This indicates the presence of a 5' untranslated RNA and a fairly long 3' extension. The promoter sequence of this gene is 5'TATAAT3' at –10, and 5'TTGACG3' at –35, a typical E. coli-like promoter sequence, and is transcribed in vitro specifically only RNA polymerase containing {sigma}55 and not {sigma}37-containing holoenzyme.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.