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Nucleic Acids Research, 1984, Vol. 12, No. 4 1875-1887
© 1984

MOLECULAR BIOLOGY

Cloning and nucleotide sequence of the simian rotavirus gene 6 that codes for the major inner capsid protein

Mary K. Estes*, Bruce B. Mason+ and Jean Cohen§

Departments of Virology and Epidemiology Baylor College of Medicine, Houston, TX 77030, USA *Department of Medicine Baylor College of Medicine, Houston, TX 77030, USA

Received December 8, 1983. Revised January 19, 1984. Accepted January 19, 1984.

The nucleotide sequence of the gene that codes for the major inner capsid protein of the simian rotavirus SAll has been determined. A DNA copy of mRNA from gene 6 was cloned in the E. coli plasmid pBR322. The full-length gene is 1357 nucleotides long with a 5'-noncoding region of 23 nucleotides and a 3'-noncoding region of 140 nucleotides. The gene contains a single, long, open reading-frame of 1194 nucleotides capable of coding for a protein of 397 amino acids with a molecular weight of 44,816. The predicted protein product is relatively proline-rich with a net charge at neutral pH of –3.5. One stretch of 53 amino acids (encoded by nucleotides 327-485) is basic.

+Present address: Microbiology Division, Wyeth Laboratories, Philadelphia, PA 19101, USA

§Present address: Station de Virologie et D'Immunologie INRA, Route de Thiverval, 78850 Thiverval-Grignon, France


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