Skip Navigation

This Article
Right arrow Print PDF (5295K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (32)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Andres, A.-C.
Right arrow Articles by Ryffel, G. U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Andres, A.-C.
Right arrow Articles by Ryffel, G. U.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1984, Vol. 12, No. 5 2283-2302
© 1984

MOLECULAR BIOLOGY

Persistence, methylation and expression of vitellogenin gene derivatives after injection into fertilized eggs of Xenopus laevis

Anne-Catherine Andres1, Daniel B. Muellener and Gerhart U. Ryffel2

Zoological Institute, Division of Cell and Developmental Biology, University of Bern, Baltzerstrasse 4, CH-3012 Bern, Switzerland

Received January 4, 1984. Accepted February 8, 1984.

We report the fate of different derivatives of the vitellogenin genes after injection into fertilized eggs of Xenopus. We injected a constructed minigene as well as a 5' fragment of the A2 vitellogenin gene. The minigene survives in embryogenesis much better than the 5' A2 fragment and is retained more frequently and at a higher level in frog tissues. The mosaic distribution of the foreign DNA an different frog tissues indicates that no integration occurred before the first cleavage stage. The persisting DNA may be partially integrated but is mostly found in an episome-like form. This unintegrated form is not supercoiled and is rearranged. Methylation of the Hpa II sites prior to injection has no influence on the survival of the injected sequences and the Hpa II sites of the surviving DNA are unmethylated irrespective whether the injected DNA was methylated or not. Whereas the derivatives are transcribed in embryos, they cannot be activated by estrogen in the liver of young frogs.

Present address: Ludwig Institute for Cancer Research, Inselspital, CH-30l0 Berne

Present address: Institute for Genetics and Toxicology, Kernforschungszentrum Karisruhe GmbH, D-7500 Karisruhe, W. Germany


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 EndocrinologyHome page
D. Crews and J. A. McLachlan
Epigenetics, Evolution, Endocrine Disruption, Health, and Disease
Endocrinology, June 1, 2006; 147(6): s4 - s10.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
J. A. McLachlan
Environmental Signaling: What Embryos and Evolution Teach Us About Endocrine Disrupting Chemicals
Endocr. Rev., June 1, 2001; 22(3): 319 - 341.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
P Pierandrei-Amaldi, I Bozzoni, and B Cardinali
Expression of the gene for ribosomal protein L1 in Xenopus embryos: alteration of gene dosage by microinjection.
Genes & Dev., January 1, 1988; 2(1): 23 - 31.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.