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Nucleic Acids Research, 1984, Vol. 12, No. 5 2327-2341
© 1984

MOLECULAR BIOLOGY

Analysis of a eukaryotic ß-galactostdase gene: the N-terminal end of the yeast Kluyveromyces lactis protein shows homology to the Escherichia coli lacZ gene product

Karin D. Breunig, Ulrike Dahlems, Sunil Das and Cornelis P. Hollenberg

Institut fur Mikrobiologie der Universitt Düsseldorf, Universittsstrasse 1, 4000 Düsseldorf 1, FRG

Received December 23, 1983. Accepted February 14, 1984.

The LAC4 gene of Kluyveromyces lactis, encoding the enzyme ß-galactosidase was mapped on a cloned DNA fragment and the sequence of the 5' end was determined. This sequence includes the 5' regulatory region involved in the induction by lactose and the N-terminal end of the protein coding region. Comparison of the deduced amino acid sequence of this eukaryotic enzyme with the N-terminal end of the Escherichia coil ß-galactosidase revealed substantial homology. Two major RNA initiation sites were mapped at -115 and -105. A number of structural peculiarities of the 5'non-coding region are discussed as in comparison to Saccharomyces cerevisiae genes.


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