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Nucleic Acids Research, 1984, Vol. 12, No. 5 2421-2426
© 1984

MOLECULAR BIOLOGY

Physical mapping of the ribosomal RNA genes of Mycoplasma capricolum

Gad Glaser*, Dorit Amikam+ and Shmuel Razin+

*Departments of Cellular Biochemistry, The Hebrew University-Hadassah Medical School Jerusalem, Israel +Membrane and Ultrastructure Research, The Hebrew University-Hadassah Medical School Jerusalem, Israel

Received January 31, 1984. Accepted February 17, 1984.

Physical mapping of the rRNA genes of Mycoplasma capricolum was done by digestion of the mycoplasmal DNA with EcoRI, PstI and Bg1II and hybridization with nick-translated probes consisting of defined portions of the rrnB ribosomal RNA operon of Escherichia coli. The results indicate that the rRNA genes in the chromosome of M. capricolum are arranged in two clusters, each organized in the order 5'-16S-23S-5S-3', resembling the ordar of the genes in the rrnB operon, with no large spacer regions separating the genes in each cluster.


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