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Nucleic Acids Research, 1984, Vol. 12, No. 5 2543-2547
© 1984

MOLECULAR BIOLOGY

The primary structures of two arginine tRNAs (anticodous C-C-U and mcm5a2U-C-{psi}) and of glutamine tRNA (anticodon C-U-G) from bovine liver

G. Keith

Institut de Biologie Moléculaire et Cellulaire du CNRS 15 rue Descartes, 67084 Strasbourg Cedex, France

Received November 16, 1983. Accepted February 1, 1984.

The primary structure of two arginine tRNAs (anticodons: C-C-U and mcm5s2U-C-{psi}) and of a glutamine tRNA (anticodon : C-U-G) of bovine liver have been determined using [32]P postlabeling methods.

They are tRNAArgCCU : pG-C-C-C-C-A-G-U-m1G-m2G-C-C-U-A-A-D-G-G-A-D-A-A-G-G-C-A-{psi}-U-G-G-C-m3C-U-C-C-U-m1G-A-G-C-C-A-G-G-G-A-D-U-G-{psi}-G-G-G-T-{psi}-C-G-m1A-G-U-C-C-C-A-U-C-U-G-G-G-G-{psi}-C-C-C-A.

tRNAArgmcm5s2UC{psi}: pG-G-C-U-C-C-G-U-m1G-G-C-G-C-A-A-D-G-G-A-D-A-G-C-G-C-A-{psi}-{psi}-G-G-A-m3C-U-mcm5s2U-C-{psi}-m1G-A-U-{psi}-C-A-A-A-G-m7G-D-U-m5C-C-G-G-G-T-({psi})-{psi}-C-G-m1A-G-U-C-C-C-G-G-C-G-G-A-G-U-C-G-C-C-A

tRNAGICUG : pG-G-U-U-C-C-A-U-m1G-G-U-G-{psi}-A-A-D-Gm-G-D-D-A-G-C-A-C-U-C-{psi}-G-G-A-Cm-U-C-U-G-A-A-{psi}-C-C-A-G-C-G-A-U-m5C-C-G-A-G-{psi}-{psi}-C-A-m1A-A-U-C-U-C-G-G-U-G-G-A-A-C-C-U-C-C-A.


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