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Nucleic Acids Research, 1984, Vol. 12, No. 6 2619-2628
© 1984

ENZYMOLOGY

Three new restriction endonucleasesMaeI, MaeII and MaeIII from Methanococcus aeolicus

Kurt Schmid1, Michael Thomm1, Axel Laminet1, Frank G. Laue2, Christoph Kessler2, Karl O. Stetter1,+ and Rüdiger Schmitt1,+

1Institut für Biochemie, Genetik und Mikrobiologie, Universitat Regensburg D-8400 Regensburg 2Boehringer Mannheim GmbH, Biochemical Research Center D-8132 Tutzing, FRG

+To whom correspondence should be addressed

Received January 31, 1984. Accepted February 20, 1984.

Three type II restriction endonucleases, MaeI, MaeII and MaeIII, with novel site specificities have been isolated and purified from the archaebacterium Methanococcus aeolicus PL-15/H. The recognition sequences of these enzymes are Formula with the sites of cleavage as indicated by the arrows. The sequences were confirmed by restriction and computer analyses on sequenced DNA's of plasmid pBR322, bacteriophages {lambda} and ØX174 and virus SV4O.


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Int J Syst Evol Microbiol, July 1, 2006; 56(Pt 7): 1525 - 1529.
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