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Nucleic Acids Research, 1984, Vol. 12, No. 6 2629-2640
© 1984

MOLECULAR BIOLOGY

Kinetics of messenger accumulation coding for IFN {gamma}, related to modifications in the poly(A) RNA population of activated human lymphocytes

Catherine Vaquero*, Josiane Sanceau*, Paul Sondermeyer+ and Rébéca Falcoff*

*Unite INSERM 196, Institut Curie, Section de Biologie 26, rue d'Ulm, 75231 Paris Cedex 05 +Transgène S.A 11, rue de Molsheim, 67000 Strasbourg, France

Received January 30, 1984. Accepted February 27, 1984.

Exposure of human lymphocytes to a mitogen induces the appearance of newly synthesized RNAs and proteins. This study describes the changes in overall synthesis as measured by pulse labelling of PHA treated lymphocytes as well as a qualitative analysis of the protein synthetic patterns "in vivo" and "in vitro". Both the levels of RNA and protein synthesis increase drastically in PHA stimulated cells, while cultures incubated without mitogen remained at background levels. The low translational activity in control cells is not due to the absence of messengers since the extracted RNAs clearly direct the synthesis of high molecular weight proteins when translated "in vitro". A number of qualitative differences are seen in the "in vitro" translation of RNA extracted from induced and non-induced lymphocytes, although the apparent protein synthetic pattern "in vivo" remains identical. The secretion of IFN-{gamma} is one of the newly expressed functions in stimulated lymphocytes and therefore has been studied more detailed in a time-course of the messenger level compared to the secreted activity of the medium. A specific probe was used to quantitate in Northern blot's the accumulation of mRNA coding for IFN- {gamma}.


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