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Nucleic Acids Research, 1984, Vol. 12, No. 6 2731-2743
© 1984

MOLECULAR BIOLOGY

Amplified DNA in Y1 mouse adrenal tumor cells: isolation of cDNAs complementary to an amplified c-Ki-ras gene and localization of homologous sequences to mouse chromosome 6

Donna L. George1,*, Alan F. Scott2, Bérengère de Martinville3 and Uta Francke3

1Dept. Human Genetics, Univ. Pennsylvania School of Medicine Philadelphia, PA 19104 2Dept. Medicine, The John Hopkins Univ. School of Medicine Baltimore, MD 21205 3Dept. Human Genetics, Yale Univ. School of Medicine 333 Cedar Street, New Haven, CT 06510, USA

*To whom correspondence should be addressed

Received December 19, 1983. Accepted February 22, 1984.

We have isolated cDNA clones complementary to a c-Ki-ras cellular oncogene that is amplified in Yl mouse adrenal tumor cells, with the amplified sequences located on double-minute chromatin bodies (DMs) and homogeneously staining chromosomal regions (HSRs). Characterization of the cDNAs included the isolation of corresponding genomic clones, Northern blot analysis of RNA, and DNA sequence analysis. Our studies demonstrate that the c-Ki-ras gene amplified in the Yl cells is homologous to the human c-Ki-ras2 gene. We have also obtained evidence that, in addition to c-Ki-ras, at least one other transcription unit has been amplified in the mouse adrenal tumor cells. Moreover, by Southern blot analysis of Chinese hamster-mouse somatic cell hybrids, we have determined that the amplified DNA sequences associated with DMs and HSRs, including the c-Ki-ras gene, are present in normal mouse cells on chromosome 6


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