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Nucleic Acids Research, 1984, Vol. 12, No. 6 2791-2805
© 1984

MOLECULAR BIOLOGY

Use of a cDNA recombinant for the {gamma}-subunit of mouse nerve growth factor to localize members of this multigene family near the TAM-1 locus on chromosome 7

Philip N. Howles, Douglas P. Dickinson, Laura L. DiCaprio, Mary Woodworth-Gutai* and Kenneth W. Gross

Department of Molecular Biology, Roswell Park Memorial Institute 666 Elm Street, Buffalo, NY 14263, USA *Department of Cell and Tumor Biology, Roswell Park Memorial Institute 666 Elm Street, Buffalo, NY 14263, USA

Received December 12, 1983. Revised February 24, 1984. Accepted February 24, 1984.

The {gamma}-subunit of mouse 7S nerve growth factor ({gamma}-NGF) is a member of a family of closely related serine proteases that includes kallikreins and tamases. We have isolated from a DBA/2J male submaxillary gland cDNA library a clone, pSM676, which codes for {gamma}-NGF. Sequence analysis of the clone shows that it codes for the C-terminal 138 amino acids of the protein plus 23 bases of the 3'-nontranslated portion of the message. The predicted amino acid sequence agrees with that determined by Thomas et al. (1) for the {gamma}-subunit of nerve growth factor from Swiss Webster mice except for the single, conservative substitution of glutamate for aspartate at amino acid 175. When used as a probe for Southern blot analysis, pSM676 hybridizes to at least twelve fragments of restricted mouse genomic DNA which correspond to several different serine protease genes. Using mouse-hamster hybrid cell lines and recombinant inbred strains of mice, we have demonstrated that all of the genes which show homology to pSM676 are located on mouse chromosome 7, clustered at or near the Tam-1 locus


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