Skip Navigation

This Article
Right arrow Print PDF (3088K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (56)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Ponzetto-Zimmerman, C.
Right arrow Articles by Wolgemuth, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ponzetto-Zimmerman, C.
Right arrow Articles by Wolgemuth, D. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1984, Vol. 12, No. 6 2807-2822
© 1984

MOLECULAR BIOLOGY

Methylation of satellite sequences in mouse spermatogenic and somatic DNAs

Carola Ponzetto-Zimmerman and Debra J. Wolgemuth

Department of Human Genetics and Development and The Center for Reproductive Sciences, Columbia University, College of Physicians and Surgeons 630 West 168th Street, New York, NY 10032, USA

Received December 8, 1983. Revised February 13, 1984. Accepted February 13, 1984.

The distribution of 5-methyl cytosine (5-MeC) residues in a highly repetitive sequence, mouse major satellite, was examined in germinal versus somatic DNAs by digestion with the methylation sensitive isoschizomers Msp I and Hpa II and Southern blot analysis, using a cloned satellite probe. DNA from liver, brain, and a mouse fibroblast cell line, C3H 10T1/2, yielded a multimeric hybridization pattern after digestion with Msp I (and control Eco RI) but were resistant to digestion with Hpa II, reflecting a high level of methylation of the satellite sequences. In contrast, DNA from mature sperm was undermethylated at these same sequences as indicated by the ability of Hpa II to generate a multimeric pattern. DNAs from purified populations of testis cells in different stages of spermatogenesis were examined to determine when during germ cell differentiation the undermethylation was established. As early as in primitive type A, type A, and type B spermatogonia, an undermethylation of satellite sequences was observed. This suggests that this highly specific undermethylation of germ cell satellite DNA occurs very early in the germ cell lineage, prior to entry into meiosis


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum Mol GenetHome page
S. Yehezkel, Y. Segev, E. Viegas-Pequignot, K. Skorecki, and S. Selig
Hypomethylation of subtelomeric regions in ICF syndrome is associated with abnormally short telomeres and enhanced transcription from telomeric regions
Hum. Mol. Genet., September 15, 2008; 17(18): 2776 - 2789.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
I. Suetake, D. Hayata, and S. Tajima
The Amino-Terminus of Mouse DNA Methyltransferase 1 Forms an Independent Domain and Binds to DNA with the Sequence Involving PCNA Binding Motif
J. Biochem., December 1, 2006; 140(6): 763 - 776.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
C. Allegrucci, A. Thurston, E. Lucas, and L. Young
Epigenetics and the germline
Reproduction, February 1, 2005; 129(2): 137 - 149.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
T. Ueda, K. Yamazaki, R. Suzuki, H. Fujimoto, H. Sasaki, Y. Sakaki, and T. Higashinakagawa
Parental methylation patterns of a transgenic locus in adult somatic tissues are imprinted during gametogenesis
Development, December 1, 1992; 116(4): 831 - 839.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
T Kafri, M Ariel, M Brandeis, R Shemer, L Urven, J McCarrey, H Cedar, and A Razin
Developmental pattern of gene-specific DNA methylation in the mouse embryo and germ line.
Genes & Dev., May 1, 1992; 6(5): 705 - 714.
[Abstract] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
R. Jaenisch, M. Breindl, K. Harbers, D. Jahner, and J. Lohler
Retroviruses and Insertional Mutagenesis
Cold Spring Harb Symp Quant Biol, January 1, 1985; 50(0): 439 - 445.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.