Nucleic Acids Research, 1984, Vol. 12, No. 7 3069-3088
© 1984
MOLECULAR BIOLOGY |
The bacteriophage 
O and P protein initiators promote the replication of single-stranded DNA
Department of Biochemistry, School of Hygiene and Public Health, Johns Hopkins University 615 North Wolfe Street, Baltimore, MD 21205, USA
Received January 19, 1984. Accepted March 12, 1984.
A soluble enzyme system that specifically initiates 
dy plasmid DNA replication at a bacteriophage replication origin [Wold et al. (1982) Proc. Natl. Acad. Sci. USA 79, 6176–6180] is also capable of replicating the single–stranded circular chromosomes of phages M13 and øX174 to a duplex form. This chain initiation on single–stranded templates is novel in that it is absolutely dependent on the O and P protein chromosomal initiators and on several Escherichia coli proteins that are known to function in the replication of the chromosome in vivo, including the host dnaB, dnaG (primase), dnaJ and dnaK replication proteins. Strand initiation occurs at multiple sites following an O and P protein–dependent pre–priming step in which the DNA is converted into an activated nucleoprotein complex containing the bacterial dnaB protein. We propose a scheme for the initiation of DNA synthesis on single–stranded templates in this enzyme system that may be relevant to strand initiation events that occur during replication of phage in vivo.
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