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Nucleic Acids Research, 1984, Vol. 12, No. 7 3115-3126
© 1984

CHEMISTRY

Comparative structural analysis of eubacterial 5S rRNA by oxidation of adenines in the N-1 position

Tomas Pieler, Angela Schreiber and Volker A. Erdmann

Institut für Biochemie, Fachbereich Chemie, Freie Universität Berlin Thielallee 69-73, 1000 Berlin 33 (Dahlem), FRG

Received January 6, 1984. Revised March 16, 1984. Accepted March 16, 1984.

Adenines in free 5s rRNA from Escherichia coli, Bacillus stearothermophilus and Thermus thermophilus have been oxidized at their N–1 position using monoperphthalic acid. The determination of the number of adenine 1–N–oxides was on the basis of UV spectroscopic data of the intact molecule. Identification of the most readily accessible nucleotides by sequencing gel analysis reveals that they are located in conserved positions within loops, exposed hairpin loops and single-base bulge loops. Implications for the structure and function of 5S rRNA will be discussed on the basis of this comparative analysis.


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