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Nucleic Acids Research, 1984, Vol. 12, No. 7 3185-3200
© 1984

MOLECULAR BIOLOGY

Association of an RNA polymerase III transcription factor with a ribonucleoprotein complex recognized by autoimmune sera

Joel M. Gottesfeld, Deborah L. Andrews and Sallie O. Hoch*

Division of Cellular Biology, Research Institute of Scripps Clinic 10666 North Torrey Pines Road *Agouron Institute 505 Coast Blvd. South, La Jolla, CA 92037, USA

Received November 21, 1983. Revised February 28, 1984. Accepted March 1, 1984.

RNA polymerase III transcription can be inhibited in vitro by two sera from patients with autoimmune diseases. The first serum, designated anti-SS-B (or La), has antibodies directed against a 50,000 dalton polypeptide that is part of a larger ribonucleoprotein complex. The second serum, designated anti-SpNo, recognizes at a target antigen polypeptide of greater than 100,000 daltons as well as the SS-B antigen. Both sera selectively remove required transcription factors from the transcription extract, and inhibition can be rescued by the addition of a HeLa S100 extract to the depleted transcription system. The HeLa S100 extract was sequentially fractionated by ion-exchange chromatography on DEAE-cellulose and phosphocellulose. The high salt eluate from the latter column was also able to rescue the anti-SS-B inhibition as was the immunoaffinity-purified SS-B ribonucleoprotein complex isolated from HeLa, Xenopus or rabbit thymus. Immunoblots of the active fractions indicated that all contained the SS-B immunoreactive polypeptide, but probes o f replica filters for DNA-binding suggested that the transcription factor is not the SSB antigen but a 64,000 dalton polypeptide component of the antigen ribonucleoprotein complex. SS-B is itself an RNA-binding protein and could be shown to bind nascent 5S RNA transcripts in vitro. Differential ammonium sulfate precipitation and DNA cellulose chromatography has confirmed that a group of 64-68 K dalton polypeptides are components of the SS-B ribonucleoprotein complex associated with transcription factor activity.


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