Nucleic Acids Research, 1984, Vol. 12, No. 7 3283-3293
© 1984
MOLECULAR BIOLOGY |
U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle
Department of Molecular Biophysics and Biochemistry, Yale University New Haven, CT 06510, USA
Received January 17, 1983. Revised March 1, 1984. Accepted March 1, 1984.
U4 and U6 RNAs of mammalian cells possess extensive intermolecular sequence complementarity and hence have the potential to base pair. A U4/U6 RNA complex, detectible in nondenaturi ng polyacrylamide gels, is released when human small nuclear ribonucleoproteins (snRNPs) containing U1, U2, U4, U5, and U6 RNAs are dissociated with proteinase K in the presence of sodium dodecyl sulfate. The released RNA/RNA complex dissociates with increasing temperature, consistent with the existence of specific base-pairing between the two RNAs. Since U6 RNA is selectively released from intact snRNPs under the same conditions required to dissociate the U4/U6 RNA complex, the RNA-RNA interaction may be sufficient to maintain U4 and U6 RNAs in the same snRNP particle. The biological implications of these findings are discussed.
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