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Nucleic Acids Research, 1984, Vol. 12, No. 7 3321-3332
© 1984

MOLECULAR BIOLOGY

Initiation signals for complementary strand DNA synthesis in the region of the replication origin of the Escherichia coli chromosome

Antoine R. Stuitje, Peter J. Weisbeek* and Michiel Meijer

Department of Electron Microscopy and Molecular Cytology, University of Amsterdam Plantage Muidergracht 14, 1018 TV Amsterdam *Department of Molecular Cell Biology, State University of Utrecht Padualaan 8, 3584 CH Utrecht, The Netherlands

Received January 10, 1984. Revised March 15, 1984. Accepted March 15, 1984.

We have used an in vivo plasmid-{Phi}X174 packaging system to detect replication initiation signals in the region of the replication origin (oriC) of the Escherichia coli chromosome. The results obtained are summarized as follows: (i) Neither within nor close to oriC effective signals for initiating complementary strand synthesis could be detected. We conclude that initiation mechanisms for leading and lagging strand synthesis at oriC are not identical to any known priming mechanism of DNA synthesis. (ii) At least five signals that can function as complementary strand origins on ss-plasmid DNA are located in a region about 2000-3300 base pairs away from oriC in the clockwise direction on the chromosome. We suggest that these signals are protein n' like recognition sequences since they are dependent for their activity on dnaB protein and show sequence similarities to other putative n' recognition sequences. Surprisingly, some of the signals are located on the template for leading strand synthesis.


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